I don't know if I'll be able to help, but let me ask a few questions...
What version of Studio are you using?
Is that the same version as before, or did you do a Studio upgrade?
Have you started a new project, or are you working with an existing project only? If so, delete auxiliary files and start a new project.
If old footage used to work, but no longer works, even in a new project (with no transitions or effects, just a plain video clip) then I have to ask WHAT CHANGED? New video card? New sound card? Other software installed? Any thing else you can think of that might have changed?
It MIGHT be possible that a "repair installation" of Studio might shake it up and get it back to normal.
This blast of questions in a normal first step in trouble shooting. We have to narrow it down from an infinite number of possibilities to merely a huge number of possibilities.
Hopefully we'll get you functional again.